Blueberry Extract for Hep C: Lloyd Wright, Author of Hepatitis C: Guide for Health, Blueberry Extract Slows Replication of Hep C Virus in Patients
- 11 years ago
Lloyd Wright, author of the just released Hepatitis C: A Do-It Yourself Guide for Health, talking about Blueberry Extract, an effective agent for preventing or slowing down the replication of the Hepatitis C Virus. Hepatitis C virus (HCV) infection is a major cause of chronic liver disease such as chronic hepatitis, cirrhosis, and hepatocellular carcinoma. While searching for new natural anti-HCV agents in agricultural products, we found a potent inhibitor of HCV RNA expression in extracts of blueberry leaves when examined in an HCV subgenomic replicon cell culture system.
This activity was observed in a methanol extract fraction of blueberry leaves and was purified by repeated fractionations in reversed-phase high-performance liquid chromatography. The final purified fraction showed a 63-fold increase in specific activity compared with the initial methanol extracts and was composed only of carbon, hydrogen, and oxygen. Liquid chromatography/mass-ion trap-time of flight analysis and butanol-HCl hydrolysis analysis of the purified fraction revealed that the blueberry leaf-derived inhibitor was proanthocyanidin.
Furthermore, structural analysis using acid thiolysis indicated that the mean degree of polymerization of the purified proanthocyanidin was 7.7, consisting predominantly of epicatechin. Proanthocyanidin with a polymerization degree of 8 to 9 showed the greatest potency at inhibiting the expression of subgenomic HCV RNA. Purified proanthocyanidin showed dose-dependent inhibition of expression of the neomycin-resistant gene and the NS-3 protein gene in the HCV subgenome in replicon cells.
While characterizing the mechanism by which proanthocyanidin inhibited HCV subgenome expression, we found that heterogeneous nuclear ribonucleoprotein A2/B1 showed affinity to blueberry leaf-derived proanthocyanidin and was indispensable for HCV subgenome expression in replicon cells. These data suggest that proanthocyanidin isolated from blueberry leaves may have potential usefulness as an anti-HCV compound by inhibiting viral replication.
Wright knows about the rigors of life with Hepatitis C, and he knows all about wandering in the dark when doctors have no answers. Wright describes the new book, which follows his international best seller Triumph Over Hepatitis C, as "having all the primary information that everyone infected with Hepatitis C must know about. The 200-million people worldwide, about 5 million of them American, can benefit tremendously from Lloyds personal experience with over 200.000 clients who have hep c, most of them having used interferon and had it fail.
Lloyd Wright's Hepatitis C book builds on the years of work already undertaken and brings valuable new information about ways to combat the destructive virus, Hepatitis C. Hepatitis C: A Do-It Yourself Guide for Health begins by chronicling Wright's own personal horror story after a 1979 accident brought Hepatitis into his life through a hospital blood transfusion. Following 15 years of pain, suffering and uncertainty he was accidently diagnosed and basically told to get his affairs in order. He refused to accept the fate prescribed to him by doctors and battled against the odds, and he prevailed. To those facing Hep C, Lloyd Wright offers these words of wisdom: "You can, and must, take responsibility for your health. If you are suffering from hepatitis C, you can reclaim your health, get out of bed, get off the couch, forget about disability, and go back to work."
A Time of Enlightenment (see Table of Contents below), Wright gives the exact supplement regimen followed in what he calls "my own recovery from Hepatitis C." The supplements, vitamins and herbs taken were "a treatment strategy" that he lays out in Hepatitis C: A Do-It Yourself Guide for Health. A few of the items used were NatCell Thymus, Non-Pasteurized Aloe and Blueberry leaf extract.
One of the new items, Naringenin currently offered by Wright is described in the book as a "powerful flavonoid" found in grapefruit that "shows promise in helping to combat hepatitis C. Naringenin is currently being used for a Phase 1 trial at UCLA. The hepatitis C virus is bound to a very low intensity lipo-protein (one of the so-called "bad" cholesterols), when it is secreted from liver cells, according to a February 4, 2007, article published in Science Daily. Researchers at the Massachusetts General Hospital Center for Engineering in Medicine reported that the viral secretion required to pass infection to other cells can be blocked by the common flavonoid Naringenin."
Lloyd Wright in Chapter Two, Hepatitis and Interferon, describes the nature of Hepatitis and the various forms in which it exists, meaning Hepatitis A, B and C, before offering this warning at the end of the section: "If you have Hepatitis C, your medical doctor will prescribe interferon. Before you proceed, read this book."
For more about Lloyd Wright go to http://alternativemedicinesolution.com
This activity was observed in a methanol extract fraction of blueberry leaves and was purified by repeated fractionations in reversed-phase high-performance liquid chromatography. The final purified fraction showed a 63-fold increase in specific activity compared with the initial methanol extracts and was composed only of carbon, hydrogen, and oxygen. Liquid chromatography/mass-ion trap-time of flight analysis and butanol-HCl hydrolysis analysis of the purified fraction revealed that the blueberry leaf-derived inhibitor was proanthocyanidin.
Furthermore, structural analysis using acid thiolysis indicated that the mean degree of polymerization of the purified proanthocyanidin was 7.7, consisting predominantly of epicatechin. Proanthocyanidin with a polymerization degree of 8 to 9 showed the greatest potency at inhibiting the expression of subgenomic HCV RNA. Purified proanthocyanidin showed dose-dependent inhibition of expression of the neomycin-resistant gene and the NS-3 protein gene in the HCV subgenome in replicon cells.
While characterizing the mechanism by which proanthocyanidin inhibited HCV subgenome expression, we found that heterogeneous nuclear ribonucleoprotein A2/B1 showed affinity to blueberry leaf-derived proanthocyanidin and was indispensable for HCV subgenome expression in replicon cells. These data suggest that proanthocyanidin isolated from blueberry leaves may have potential usefulness as an anti-HCV compound by inhibiting viral replication.
Wright knows about the rigors of life with Hepatitis C, and he knows all about wandering in the dark when doctors have no answers. Wright describes the new book, which follows his international best seller Triumph Over Hepatitis C, as "having all the primary information that everyone infected with Hepatitis C must know about. The 200-million people worldwide, about 5 million of them American, can benefit tremendously from Lloyds personal experience with over 200.000 clients who have hep c, most of them having used interferon and had it fail.
Lloyd Wright's Hepatitis C book builds on the years of work already undertaken and brings valuable new information about ways to combat the destructive virus, Hepatitis C. Hepatitis C: A Do-It Yourself Guide for Health begins by chronicling Wright's own personal horror story after a 1979 accident brought Hepatitis into his life through a hospital blood transfusion. Following 15 years of pain, suffering and uncertainty he was accidently diagnosed and basically told to get his affairs in order. He refused to accept the fate prescribed to him by doctors and battled against the odds, and he prevailed. To those facing Hep C, Lloyd Wright offers these words of wisdom: "You can, and must, take responsibility for your health. If you are suffering from hepatitis C, you can reclaim your health, get out of bed, get off the couch, forget about disability, and go back to work."
A Time of Enlightenment (see Table of Contents below), Wright gives the exact supplement regimen followed in what he calls "my own recovery from Hepatitis C." The supplements, vitamins and herbs taken were "a treatment strategy" that he lays out in Hepatitis C: A Do-It Yourself Guide for Health. A few of the items used were NatCell Thymus, Non-Pasteurized Aloe and Blueberry leaf extract.
One of the new items, Naringenin currently offered by Wright is described in the book as a "powerful flavonoid" found in grapefruit that "shows promise in helping to combat hepatitis C. Naringenin is currently being used for a Phase 1 trial at UCLA. The hepatitis C virus is bound to a very low intensity lipo-protein (one of the so-called "bad" cholesterols), when it is secreted from liver cells, according to a February 4, 2007, article published in Science Daily. Researchers at the Massachusetts General Hospital Center for Engineering in Medicine reported that the viral secretion required to pass infection to other cells can be blocked by the common flavonoid Naringenin."
Lloyd Wright in Chapter Two, Hepatitis and Interferon, describes the nature of Hepatitis and the various forms in which it exists, meaning Hepatitis A, B and C, before offering this warning at the end of the section: "If you have Hepatitis C, your medical doctor will prescribe interferon. Before you proceed, read this book."
For more about Lloyd Wright go to http://alternativemedicinesolution.com